Defender Jones and goalkeeper Romero were both also left out of United’s Champions League squad earlier this month after falling out of favour under manager Ole Gunnar Solskjaer.
Their omissions cast doubt over their long-term future at Old Trafford.
Phil Jones last played for Manchester United in January and has been injured since June
Romero, 33, has entered the last year of his contract and has wanted to leave the club since he was dropped for the Europa League semi-final defeat to Sevilla on August 16.
Jones last played for United in January and has been injured since June but the 28-year-old has a contract until 2023.
Before Tuesday’s Champions League tie at PSG, Solskjaer declined to say whether he would consider picking Jones or Romero in the future.
Sergio Romero, 33, has entered the last year of his contract and wants to leave the club
‘We’ve got a squad of 25 to put on the list,’ Solskjaer said.
‘Phil has been injured. Sergio has not come back to us yet.’
Defender Marcos Rojo, who is back at United following his loan spell with Estudiantes, is included in the squad along with new deadline-day signings Alex Telles and Edinson Cavani.