Defender Jones and goalkeeper Romero were both also left out of United’s Champions League squad earlier this month after falling out of favour under manager Ole Gunnar Solskjaer.
Their omissions cast doubt over their long-term future at Old Trafford.
Phil Jones last played for Manchester United in January and has been injured since June
Sergio Romero, 33, has entered the last year of his contract and wants to leave the club
Marcos Rojo, who is back at United following his loan spell with Estudiantes, is included in the squad along with new deadline-day signings Alex Telles and Edinson Cavani.